Article date: April 1976
By: A.H. DRUMMOND, J.L. GORDON in Volume 56, Issue 4, pages 417-421
The uptake of 5‐hydroxytryptamine (5‐HT) by rat blood platelets in citrated plasma was linear for at most 10 s and was substantially complete within 3 minutes.
Adenosine 5′‐diphosphate (ADP) was a potent inhibitor of 5‐HT uptake (Ki = 0.38 μm) and kinetic analysis revealed that the inhibition was not competitive.
Inhibition of 5‐HT uptake by ADP was abolished in the presence of prostaglandin E1 and 2‐n‐amylthio‐AMP, which also inhibit the stimulant actions of ADP on blood platelets.
It is concluded that ADP could inhibit 5‐HT uptake by changing the Na+/K+ distribution across the cell membrane, and the biological significance of this is discussed.
The uptake of 5‐hydroxytryptamine (5‐HT) by rat blood platelets in citrated plasma was linear for at most 10 s and was substantially complete within 3 minutes.
Adenosine 5′‐diphosphate (ADP) was a potent inhibitor of 5‐HT uptake (Ki = 0.38 μm) and kinetic analysis revealed that the inhibition was not competitive.
Inhibition of 5‐HT uptake by ADP was abolished in the presence of prostaglandin E1 and 2‐n‐amylthio‐AMP, which also inhibit the stimulant actions of ADP on blood platelets.
It is concluded that ADP could inhibit 5‐HT uptake by changing the Na+/K+ distribution across the cell membrane, and the biological significance of this is discussed.
DOI: 10.1111/j.1476-5381.1976.tb07452.x
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