Article date: April 1976
By: SANDRA M. EGAN, J.D.P. GRAHAM, M.J. LEWIS in Volume 56, Issue 4, pages 413-416
Weighed stripped vasa deferentia were incubated in Holman's solution containing (a) [14C]‐sorbitol 0.014 mM, (b) [3H]‐noradrenaline ([3H]‐NA) 12.31 nM, (c) [3H]‐tetrahydrocannabinol ([3H]‐Δ1‐THC) 1 μg/ml for 5, 10, 20 and 30 minutes.
Tissues were washed, dissolved in Protosol, counted by standard scintillation counting technique and ‘drug space’ expressed as ct min−1 mg−1 tissue/ct min−1 μl−1 bathing fluid.
Vasa incubated for 30 min with [14C]‐sorbitol were washed for varying lengths of time; 82% clearance had taken place after 2 washes of 5 minutes.
The uptake of [3H]‐NA was inhibited by the presence of desmethylimipramine (DMI) 10 nM in the bath or by pretreatment of rats with 6‐hydroxydopamine (6‐OHDA).
The uptake of [3H]‐Δ1‐THC was not inhibited by the presence of DMI. It was reduced but not abolished by 6‐OHDA pretreatment.
Weighed stripped vasa deferentia were incubated in Holman's solution containing (a) [14C]‐sorbitol 0.014 mM, (b) [3H]‐noradrenaline ([3H]‐NA) 12.31 nM, (c) [3H]‐tetrahydrocannabinol ([3H]‐Δ1‐THC) 1 μg/ml for 5, 10, 20 and 30 minutes.
Tissues were washed, dissolved in Protosol, counted by standard scintillation counting technique and ‘drug space’ expressed as ct min−1 mg−1 tissue/ct min−1 μl−1 bathing fluid.
Vasa incubated for 30 min with [14C]‐sorbitol were washed for varying lengths of time; 82% clearance had taken place after 2 washes of 5 minutes.
The uptake of [3H]‐NA was inhibited by the presence of desmethylimipramine (DMI) 10 nM in the bath or by pretreatment of rats with 6‐hydroxydopamine (6‐OHDA).
The uptake of [3H]‐Δ1‐THC was not inhibited by the presence of DMI. It was reduced but not abolished by 6‐OHDA pretreatment.
DOI: 10.1111/j.1476-5381.1976.tb07451.x
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