Article date: September 1972
By: D. J. GAGNON, P. SIROIS in Volume 46, Issue 1, pages 89-93
. The antagonism of the contractile effects of prostaglandins F2α and E2 by polyphloretin phosphate (PPP) was studied on the rat isolated colon.
. PPP (20 μg/ml) was found to reduce the myotropic action of angiotensin I (10 ng/ml) and II (0·3 ng/ml) whereas larger doses of both agents were not influenced.
. It is concluded that when a mixture of antagonists is used, including PPP, the rat isolated colon can be used to assay angiotensin with accuracy and specificity. The addition of 8‐L‐ala‐ATII affords a further test of specificity.
. The antagonism of the contractile effects of prostaglandins F2α and E2 by polyphloretin phosphate (PPP) was studied on the rat isolated colon.
. PPP (20 μg/ml) was found to reduce the myotropic action of angiotensin I (10 ng/ml) and II (0·3 ng/ml) whereas larger doses of both agents were not influenced.
. It is concluded that when a mixture of antagonists is used, including PPP, the rat isolated colon can be used to assay angiotensin with accuracy and specificity. The addition of 8‐L‐ala‐ATII affords a further test of specificity.
DOI: 10.1111/j.1476-5381.1972.tb06851.x
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