INTERACTION OF NEUROTENSIN WITH THE SUBSTANCE P RECEPTOR MEDIATING HISTAMINE RELEASE FROM RAT MAST CELLS AND THE FLARE IN HUMAN SKIN

Article date: November 1982

By: J.C. FOREMAN, C.C. JORDAN, W. PIOTROWSKI in Volume 77, Issue 3, pages 531-539

Substance P induced histamine release from rat peritoneal mast cells in a dose‐dependent manner over the concentration range 1 to 10 μm.

At concentrations in the range 2.5 to 10 μm, neurotensin produced only about 5% release of histamine, which was substantially less than the maximum effect obtained with substance P.

Neurotensin, 2.5 to 10 μm produced graded inhibition of histamine release induced by substance P. The inhibitory effect of neurotensin was not seen when histamine release was induced by an antigen‐antibody reaction or by the ionophore, A 23187. Some evidence was obtained to suggest that compound 48/80 may interact with the same receptor as substance P and neurotensin.

[d‐Arg8]neurotensin, [d‐Arg9]neurotensin, xenopsin and the C‐terminal octapeptide of substance P (SP4–11) all inhibited histamine release by substance P, but physalaemin did not.

Neurotensin inhibited the wheal and flare reactions induced by substance P in human skin.

[d‐Trp7,9]substance P released histamine from rat mast cells and was about 12 times more potent than substance P itself. [d‐Trp7,9]SP1–11 also produced wheal and flare responses in human skin, being 1.8 times more potent than substance P in the production of flare.

Substance P induced histamine release from rat peritoneal mast cells in a dose‐dependent manner over the concentration range 1 to 10 μm.

At concentrations in the range 2.5 to 10 μm, neurotensin produced only about 5% release of histamine, which was substantially less than the maximum effect obtained with substance P.

Neurotensin, 2.5 to 10 μm produced graded inhibition of histamine release induced by substance P. The inhibitory effect of neurotensin was not seen when histamine release was induced by an antigen‐antibody reaction or by the ionophore, A 23187. Some evidence was obtained to suggest that compound 48/80 may interact with the same receptor as substance P and neurotensin.

[d‐Arg8]neurotensin, [d‐Arg9]neurotensin, xenopsin and the C‐terminal octapeptide of substance P (SP4–11) all inhibited histamine release by substance P, but physalaemin did not.

Neurotensin inhibited the wheal and flare reactions induced by substance P in human skin.

[d‐Trp7,9]substance P released histamine from rat mast cells and was about 12 times more potent than substance P itself. [d‐Trp7,9]SP1–11 also produced wheal and flare responses in human skin, being 1.8 times more potent than substance P in the production of flare.

DOI: 10.1111/j.1476-5381.1982.tb09328.x

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