Article date: July 1995
By: Christine Coméra, Frarçise Russo‐Marie, in Volume 115, Issue 6, pages 1043-1047
We have studied the ability of the glucocorticoid, dexamethasone, to induce annexin 1 secretion by either human blood monocytes or rat peritoneal leukocytes.
The in vivo treatment of rats with dexamethasone (1.25 mg kg−1) selectively induced secretion of annexin 1 by peritoneal leukocytes, as assessed by incubating these cells in culture medium. Annexin 1 secretion was also induced in human cultured monocytes, in vitro, by 10−6m dexamethasone.
Annexin 1 secretion was inhibited in the presence of 20 mM NH4C1 or by conducting the experiments at 18°C. In contrast, it was not inhibited by monensin, nocodazole or brefeldin A.
The time necessary for annexin 1 synthesis and secretion was less than 15 min.
These data indicate that glucocorticoids induce annexin 1 secretion by monocytes or peritoneal leukocytes. Because it is not inhibited by monensin, nocodazole or brefeldin A and it is rapid, annexin 1 secretion seems to occur by the secretory pathway similar to that used by several cytosolic proteins such as interleukin‐iβ.
We have studied the ability of the glucocorticoid, dexamethasone, to induce annexin 1 secretion by either human blood monocytes or rat peritoneal leukocytes.
The in vivo treatment of rats with dexamethasone (1.25 mg kg−1) selectively induced secretion of annexin 1 by peritoneal leukocytes, as assessed by incubating these cells in culture medium. Annexin 1 secretion was also induced in human cultured monocytes, in vitro, by 10−6m dexamethasone.
Annexin 1 secretion was inhibited in the presence of 20 mM NH4C1 or by conducting the experiments at 18°C. In contrast, it was not inhibited by monensin, nocodazole or brefeldin A.
The time necessary for annexin 1 synthesis and secretion was less than 15 min.
These data indicate that glucocorticoids induce annexin 1 secretion by monocytes or peritoneal leukocytes. Because it is not inhibited by monensin, nocodazole or brefeldin A and it is rapid, annexin 1 secretion seems to occur by the secretory pathway similar to that used by several cytosolic proteins such as interleukin‐iβ.
DOI: 10.1111/j.1476-5381.1995.tb15916.x
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