Article date: January 1991
By: P.K. Moore, A.O. Oluyomi, R.C. Babbedge, P. Wallace, S.L. Hart, in Volume 102, Issue 1, pages 198-202
l‐NG‐nitro arginine methyl ester (l‐NAME, 1–75 mg kg−1) administered intraperitoneally (i.p.) elicits dose‐related antinociception in the mouse assessed by the formalin‐induced paw licking procedure. Antinociceptive activity is still present 24 h after injection. l‐NAME (75 mg kg−1, i.p.) is also antinociceptive in the acetic acid‐induced abdominal constriction and hot plate procedures.
l‐NAME additionally produces a dose‐related inhibition of formalin‐induced paw licking following intracerebroventricular (i.c.v., 0.1–100 μg per mouse) and oral (p.o., 75–150 mg kg−1) administration.
l‐Arginine (600 mg kg−1, i.p.) but not d‐arginine (600 mg kg−1) or naloxone (5 mg kg−1) reverses the antinociceptive effect of l‐NAME in the formalin test.
High doses of l‐NAME (37.5–600 mg kg−1) but not d‐NAME (75 mg kg−1) administered i.p. produce dose‐related increases in blood pressure of the urethane‐anaesthetized mouse whilst i.c.v. injected l‐NAME (0.1 and 100 μg per mouse) is inactive.
l‐NAME (75 mg kg−1, i.p.) did not inhibit oedema formation in the formalin‐injected mouse hindpaw.
l‐NAME (75 mg kg−1) did not produce any overt behavioural changes in treated mice and failed to influence locomotor activity or the incidence of dipping, crossing, rearing or circling behaviour assessed by a modified ‘head‐dipping’ board procedure. A high dose of l‐NAME (600 mg kg−1) reduced dipping behaviour and locomotor activity suggesting a possible sedative effect. d‐NAME (600 mg kg−1) was inactive.
These results suggest that l‐NAME produces an opioid‐independent and long‐lasting antinociception in the mouse most probably by a direct effect within the central nervous system.
l‐NG‐nitro arginine methyl ester (l‐NAME, 1–75 mg kg−1) administered intraperitoneally (i.p.) elicits dose‐related antinociception in the mouse assessed by the formalin‐induced paw licking procedure. Antinociceptive activity is still present 24 h after injection. l‐NAME (75 mg kg−1, i.p.) is also antinociceptive in the acetic acid‐induced abdominal constriction and hot plate procedures.
l‐NAME additionally produces a dose‐related inhibition of formalin‐induced paw licking following intracerebroventricular (i.c.v., 0.1–100 μg per mouse) and oral (p.o., 75–150 mg kg−1) administration.
l‐Arginine (600 mg kg−1, i.p.) but not d‐arginine (600 mg kg−1) or naloxone (5 mg kg−1) reverses the antinociceptive effect of l‐NAME in the formalin test.
High doses of l‐NAME (37.5–600 mg kg−1) but not d‐NAME (75 mg kg−1) administered i.p. produce dose‐related increases in blood pressure of the urethane‐anaesthetized mouse whilst i.c.v. injected l‐NAME (0.1 and 100 μg per mouse) is inactive.
l‐NAME (75 mg kg−1, i.p.) did not inhibit oedema formation in the formalin‐injected mouse hindpaw.
l‐NAME (75 mg kg−1) did not produce any overt behavioural changes in treated mice and failed to influence locomotor activity or the incidence of dipping, crossing, rearing or circling behaviour assessed by a modified ‘head‐dipping’ board procedure. A high dose of l‐NAME (600 mg kg−1) reduced dipping behaviour and locomotor activity suggesting a possible sedative effect. d‐NAME (600 mg kg−1) was inactive.
These results suggest that l‐NAME produces an opioid‐independent and long‐lasting antinociception in the mouse most probably by a direct effect within the central nervous system.
DOI: 10.1111/j.1476-5381.1991.tb12153.x
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