Article date: October 1981
By: VA O'Neill, MD Rawlins, PH Chapman, in Volume 12, Issue 4, pages 517-521
1 Epoxide hydrolase (EH) activity was measured in biopsied skin (n = 42) using 7‐[H3]‐styrene oxide as substrate, and separation of the products by high performance liquid chromatography. 2 EH activity (mean +/‐ s.d.) was present in separated epidermis (139 +/‐ 105 pmol glycol formed mg‐1 min‐1) and dermis (165 +/‐ 120 pmol glycol formed mg‐1 microsomal protein min‐1). 3 Whole skin EH activity (mean +/‐ s.d.) varied widely (433 +/‐ 254 pmol glycol formed mg‐1 microsomal protein min‐1) 4 No significant difference in EH activity was observed in skin from breast, penis and leg. 5 Skin EH activity does not appear to contribute significantly to the systemic metabolism of epoxide, but may be important in determining the effects of epoxides formed within the epidermis.
DOI: 10.1111/j.1365-2125.1981.tb01260.x
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