Article date: January 1984
By: Margaret A. Moses, Christopher R. Snell in Volume 81, Issue 1, pages 169-174
The effect of exogenous substances on the expression of opiate receptors on 108CC15 neuroblastoma × glioma hybrid cells has been studied.
Cell differentiation by culture in the presence of N6‐O2‐dibutyryl adenosine 3′,5′‐cyclic monophosphate induced a three fold increase in opiate receptor density.
When the cells were grown in the presence of 10−5m morphine hydrochloride for up to 23 days, opiate receptor densities were reduced by only 30% when compared with matched controls.
Culture in the presence of 10−7md‐Ala2‐d‐Leu5‐enkephalin produced opiate receptor down regulation of 73% compared to controls after only 4 h of treatment.
The down regulation process could be inhibited by continued exposure to d‐Ala2d‐Leu5‐enkephalin at concentrations greater than 4 nm; below this concentration down regulation was rapid and irreversible.
A model to explain these observations is described.
The effect of exogenous substances on the expression of opiate receptors on 108CC15 neuroblastoma × glioma hybrid cells has been studied.
Cell differentiation by culture in the presence of N6‐O2‐dibutyryl adenosine 3′,5′‐cyclic monophosphate induced a three fold increase in opiate receptor density.
When the cells were grown in the presence of 10−5m morphine hydrochloride for up to 23 days, opiate receptor densities were reduced by only 30% when compared with matched controls.
Culture in the presence of 10−7md‐Ala2‐d‐Leu5‐enkephalin produced opiate receptor down regulation of 73% compared to controls after only 4 h of treatment.
The down regulation process could be inhibited by continued exposure to d‐Ala2d‐Leu5‐enkephalin at concentrations greater than 4 nm; below this concentration down regulation was rapid and irreversible.
A model to explain these observations is described.
DOI: 10.1111/j.1476-5381.1984.tb10757.x
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