Article date: February 1980
By: SHEILA A. DOGGRELL, GERALD W. SCOTT in Volume 71, Issue 2, pages 429-434
The effects of time and of indomethacin on contractile responses of the guinea‐pig gall bladder were studied in vitro.
The tissues contracted to field stimulation at 5 Hz (in the absence and presence of atropine 10−6m), (‐)‐noradrenaline (10−5m), acetylcholine (10−5m), and adenosine 5′‐triphosphate (ATP, 10−4m); the magnitude of the contractile responses increased with time.
(‐)‐Isoprenaline 10−5m either relaxed (17 of 23 preparations tested) or had no effect on gall bladder strips.
The responses of strips of guinea‐pig gall bladder to field stimulation at 5 Hz (in the absence or presence of atropine 10−6m), (‐)‐noradrenaline (10−5m), and acetylcholine (10−5m) obtained 4 h 45 min after setting up the tissue were reduced following incubation with indomethacin (7.9 times 10−6m for 1 h). The responses to (‐)‐isoprenaline (10−5m) and to ATP (10−4m) were abolished by incubation with indomethacin.
These results suggest that, in the guinea‐pig gall bladder in vitro, the magnitude of the contractile responses to field stimulation at 5 Hz, (‐)‐noradrenaline (10−5m), and acetylcholine (10−5m) and the ability of the tissue to respond to (‐)‐isoprenaline (10−5m) and ATP (10−4m), may be dependent on the synthesis of a prostaglandin‐like substance.
The effects of time and of indomethacin on contractile responses of the guinea‐pig gall bladder were studied in vitro.
The tissues contracted to field stimulation at 5 Hz (in the absence and presence of atropine 10−6m), (‐)‐noradrenaline (10−5m), acetylcholine (10−5m), and adenosine 5′‐triphosphate (ATP, 10−4m); the magnitude of the contractile responses increased with time.
(‐)‐Isoprenaline 10−5m either relaxed (17 of 23 preparations tested) or had no effect on gall bladder strips.
The responses of strips of guinea‐pig gall bladder to field stimulation at 5 Hz (in the absence or presence of atropine 10−6m), (‐)‐noradrenaline (10−5m), and acetylcholine (10−5m) obtained 4 h 45 min after setting up the tissue were reduced following incubation with indomethacin (7.9 times 10−6m for 1 h). The responses to (‐)‐isoprenaline (10−5m) and to ATP (10−4m) were abolished by incubation with indomethacin.
These results suggest that, in the guinea‐pig gall bladder in vitro, the magnitude of the contractile responses to field stimulation at 5 Hz, (‐)‐noradrenaline (10−5m), and acetylcholine (10−5m) and the ability of the tissue to respond to (‐)‐isoprenaline (10−5m) and ATP (10−4m), may be dependent on the synthesis of a prostaglandin‐like substance.
DOI: 10.1111/j.1476-5381.1980.tb10955.x
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