Article date: April 1980
By: PER LINDSTRÖM, JANOVE SEHLIN, INGE‐BERT TÄLJEDAL in Volume 68, Issue 4, pages 773-778
Transmembrane transport of 3H‐labelled 5‐hydroxytryptamine (5‐HT) by isolated pancreatic islets of non‐inbred ob/ob mice was studied.
5‐HT was vigorously accumulated in a temperature‐dependent way by the islet cells.
Studies of the concentration‐dependence of [3H]‐5‐HT uptake revealed complex kinetics with one component being saturated at 1 to 3 um 5‐HT (apparent association constant 0.6 × 106m−1) and the other non‐saturated up to 1 mm 5‐HT.
The saturable uptake was inhibited by Na+‐deficiency and metabolic poisoning with 2,4‐dinitro‐phenol and antimycin A, whereas the non‐saturable component was not affected.
Omission of K+, Ca2+ or Mg2+ did not affect the uptake rate.
It is concluded that 5‐HT is taken up by pancreatic β‐cells by mechanisms very similar to those observed in thrombocytes and neurones.
Transmembrane transport of 3H‐labelled 5‐hydroxytryptamine (5‐HT) by isolated pancreatic islets of non‐inbred ob/ob mice was studied.
5‐HT was vigorously accumulated in a temperature‐dependent way by the islet cells.
Studies of the concentration‐dependence of [3H]‐5‐HT uptake revealed complex kinetics with one component being saturated at 1 to 3 um 5‐HT (apparent association constant 0.6 × 106m−1) and the other non‐saturated up to 1 mm 5‐HT.
The saturable uptake was inhibited by Na+‐deficiency and metabolic poisoning with 2,4‐dinitro‐phenol and antimycin A, whereas the non‐saturable component was not affected.
Omission of K+, Ca2+ or Mg2+ did not affect the uptake rate.
It is concluded that 5‐HT is taken up by pancreatic β‐cells by mechanisms very similar to those observed in thrombocytes and neurones.
DOI: 10.1111/j.1476-5381.1980.tb10871.x
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