Article date: March 1969
By: M. E. J. BILLINGHAM, B. V. ROBINSON, J. M. ROBSON in Volume 35, Issue 3, pages 543-557
The carrageenin foot test was established as a sensitive and reliable assay procedure for determining the anti‐inflammatory activity of inflammatory exudates.
Incubation alone at a temperature above 70° C or with pronase at 37° C destroyed the anti‐inflammatory activity of exudate.
The anti‐inflammatory component of exudate was partially precipitated by 50% ammonium sulphate.
A partial purification process was devised using Sephadex G‐150 gel filtration and DEAE and CM cellulose ion exchange chromatography to obtain at least a 24 fold purification.
Measurements of 11‐hydroxycorticosteroid levels indicated that steroids were not involved in the mechanism by which the exudate produced its antiinflammatory effects.
The carrageenin foot test was established as a sensitive and reliable assay procedure for determining the anti‐inflammatory activity of inflammatory exudates.
Incubation alone at a temperature above 70° C or with pronase at 37° C destroyed the anti‐inflammatory activity of exudate.
The anti‐inflammatory component of exudate was partially precipitated by 50% ammonium sulphate.
A partial purification process was devised using Sephadex G‐150 gel filtration and DEAE and CM cellulose ion exchange chromatography to obtain at least a 24 fold purification.
Measurements of 11‐hydroxycorticosteroid levels indicated that steroids were not involved in the mechanism by which the exudate produced its antiinflammatory effects.
DOI: 10.1111/j.1476-5381.1969.tb08295.x
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