Article date: May 2011
By: Zhih‐Cherng Chen, Bu‐Chin Yu, Li‐Jen Chen, Kai‐Chun Cheng, Hung Jung Lin, Juei‐Tang Cheng in Volume 163, Issue 2, pages 390-398
BACKGROUND AND PURPOSE
Digoxin has been used as an inotropic agent in heart failure for a long time. Troponin I (TnI) phosphorylation is related to cardiac contractility, and the genes are regulated by peroxisome proliferator‐activated receptors (PPARs). Our previous studies indicated that cardiac abnormality related to the depressed expression of PPARδ in the hearts of STZ rats is reversed by digoxin. However, the cellular mechanisms for this effect of digoxin have not been elucidated. The aim of the present study was to investigate possible mechanisms for this effect of digoxin using the H9c2 cell line cultured in high glucose (HG) conditions.
METHODS
The effects of digoxin on PPARδ expression, intracellular calcium and TnI phosphorylation were investigated in cultured H9c2 cells, maintained in a HG medium, by using Western blot analysis.
RESULTS
Digoxin increased PPARδ expression in H9c2 cells subjected to HG conditions, and increase the intracellular calcium concentration. This effect of digoxin was blocked by BAPTA‐AM at concentrations sufficient to chelate calcium ions. In addition, the calcineurin inhibitor cyclosporine A and KN93, an inhibitor of calcium/calmodulin‐dependent protein kinase, inhibited this action. Digoxin also increased TnI phosphorylation and this was inhibited when PPARδ was silenced by the addition of RNAi to the cells. Similar changes were observed on the contraction of H9c2 cells.
CONCLUSION
The results suggest that digoxin appears, through calcium‐triggered signals, to reverse the reduced expression of PPARδ in H9c2 cells caused by HG treatment.
DOI: 10.1111/j.1476-5381.2011.01212.x
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