Article date: June 2004
By: Satoshi Akiba, Masahiro Chiba, Yuki Mukaida, Akira Tamura, Takashi Sato in Volume 142, Issue 3, pages 419-424
The leaf extract of Ginkgo Biloba L. exhibits a variety of pharmacological effects through an antioxidant action. We examined the effects of the leaf extract (Ginkgolon‐24) on the production of fibronectin induced by oxidized low‐density lipoprotein (oxLDL) in rat mesangial cells.
Stimulation with oxLDL accelerated the production of fibronectin with the preceding generation of reactive oxygen species (ROS). Pretreatment with Ginkgolon‐24 inhibited the oxLDL‐induced fibronectin production as well as ROS generation.
oxLDL also elicited the activation of SP‐1, nuclear factor‐κB, and cAMP response element‐binding protein, which are transcription factors involved in the fibronectin production. Among these activated transcription factors, Ginkgolon‐24 inhibited the activation of SP‐1 only.
Furthermore, 7‐ketocholesterol, an oxidized lipid in oxLDL particles, induced the production of fibronectin and the activation of SP‐1, which were also suppressed by Ginkgolon‐24.
These results suggest that the leaf extract of Ginkgo Biloba L. inhibits the oxLDL‐induced production of fibronectin probably through inhibitory effects on ROS generation and SP‐1 activation in rat mesangial cells.
The leaf extract of Ginkgo Biloba L. exhibits a variety of pharmacological effects through an antioxidant action. We examined the effects of the leaf extract (Ginkgolon‐24) on the production of fibronectin induced by oxidized low‐density lipoprotein (oxLDL) in rat mesangial cells.
Stimulation with oxLDL accelerated the production of fibronectin with the preceding generation of reactive oxygen species (ROS). Pretreatment with Ginkgolon‐24 inhibited the oxLDL‐induced fibronectin production as well as ROS generation.
oxLDL also elicited the activation of SP‐1, nuclear factor‐κB, and cAMP response element‐binding protein, which are transcription factors involved in the fibronectin production. Among these activated transcription factors, Ginkgolon‐24 inhibited the activation of SP‐1 only.
Furthermore, 7‐ketocholesterol, an oxidized lipid in oxLDL particles, induced the production of fibronectin and the activation of SP‐1, which were also suppressed by Ginkgolon‐24.
These results suggest that the leaf extract of Ginkgo Biloba L. inhibits the oxLDL‐induced production of fibronectin probably through inhibitory effects on ROS generation and SP‐1 activation in rat mesangial cells.
British Journal of Pharmacology (2004) 142, 419–424. doi:10.1038/sj.bjp.0705805
DOI: 10.1038/sj.bjp.0705805
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