Article date: July 1996
By: Hwee Teoh, Marzia Malcangio, Norman G. Bowery, in Volume 118, Issue 5, pages 1153-1160
The effects of various GABA receptor ligands on the electrically‐evoked release of endogenous GABA, glutamate and substance P‐like immunoreactivity from the dorsal horn of rat isolated spinal cord were examined.
Exogenous GABA (10–300 μm) significantly decreased the evoked, but not basal, release of endogenous glutamate in a concentration‐dependent manner. The GABAA agonist, isoguvacine (1–100 μm), failed to decrease the release of glutamate although it did reduce the release of GABA. Baclofen (0.1–1000 μm), the GABAB agonist, reduced the release of GABA and glutamate in a stereospecific and concentration‐dependent manner.
The actions of five GABAB antagonists on these release systems were compared. CGP36742, CGP52432, CGP55845A and CGP57250A significantly increased the evoked release of GABA and glutamate. They also reversed the effects of (−)−baclofen in a concentration‐dependent manner. On the other hand, while CGP56999A had no effect on glutamate release, it was an effective antagonist of the baclofen‐induced inhibition of GABA and substance P release.
These results suggest that GABAB receptors on nerve terminals within the dorsal horn spinal cord may be heterogeneous. However, this is based solely on the data obtained with CGP56999A which affected only GABA and substance P, but not glutamate, release.
The effects of various GABA receptor ligands on the electrically‐evoked release of endogenous GABA, glutamate and substance P‐like immunoreactivity from the dorsal horn of rat isolated spinal cord were examined.
Exogenous GABA (10–300 μm) significantly decreased the evoked, but not basal, release of endogenous glutamate in a concentration‐dependent manner. The GABAA agonist, isoguvacine (1–100 μm), failed to decrease the release of glutamate although it did reduce the release of GABA. Baclofen (0.1–1000 μm), the GABAB agonist, reduced the release of GABA and glutamate in a stereospecific and concentration‐dependent manner.
The actions of five GABAB antagonists on these release systems were compared. CGP36742, CGP52432, CGP55845A and CGP57250A significantly increased the evoked release of GABA and glutamate. They also reversed the effects of (−)−baclofen in a concentration‐dependent manner. On the other hand, while CGP56999A had no effect on glutamate release, it was an effective antagonist of the baclofen‐induced inhibition of GABA and substance P release.
These results suggest that GABAB receptors on nerve terminals within the dorsal horn spinal cord may be heterogeneous. However, this is based solely on the data obtained with CGP56999A which affected only GABA and substance P, but not glutamate, release.
DOI: 10.1111/j.1476-5381.1996.tb15518.x
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