Article date: June 1996
By: Colin D.A. Brown, Adam J. Dudley, in Volume 118, Issue 3, pages 443-444
The effects of chloride channel blockers upon intracellular pH (pHi) were examined in renal epithelial monolayers of LLC‐PK1 cells. A significant intracellular acidification was found with addition of 100 μm 5‐nitro‐2‐(3‐phenylpropylamino)‐benzoate (NPPB), niflumic acid, flufenamate and diphenylamine‐2‐carboxylate (DPC) but not with 4,4′‐diisothiocyanatostilbene‐2‐2′disulphonic acid (DIDS). The effects of these agents upon pHi was dose‐dependent with apparent K0.5 values of: 16.7±0.3 μm, 34.2±0.9 μm and 740±13 μm for niflumic acid, flufenamate and DPC respectively. The results indicate that at concentrations commonly used to block channel activity these chloride channel blockers have profound effects upon pHi.
DOI: 10.1111/j.1476-5381.1996.tb15422.x
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