Article date: October 1991
By: H.‐K. Heim, A. Oestmann, K.‐Fr. Sewing, in Volume 104, Issue 2, pages 526-530
Glycoprotein and protein synthesis in and release from pig isolated, enriched gastric mucous cells were measured by the incorporation of N‐acetyl‐[14C]‐d‐glucosamine and [3H]‐l‐leucine, respectively, into cellular and released acid precipitable material.
Histamine and activators of the adenosine 3′:5′‐cyclic monophosphate (cyclic AMP) system maximally stimulated total protein and glycoprotein synthesis in and release from the cells at concentrations of histamine (10 μm), forskolin (10–100 μm), 3‐isobutyl‐1‐methylxanthine (100 μm), and dibutyryl cyclic AMP (1–3 mm), respectively. In the presence of 3‐isobutyl‐1‐methylxanthine (30 μm) histamine stimulation was enhanced.
As shown by gel chromatography, stimulation by histamine (100 μm), forskolin (10 μm), 3‐isobutyl‐1‐methylxanthine (100 μm) and dibutyryl cyclic AMP (1 mm) resulted in a release of high molecular weight (≅2 × 106daltons) glycoproteins from the cells. The histamine H2‐receptor antagonist, ranitidine (100 μm), blocked the effect of histamine.
We conclude that cyclic AMP‐dependent processes are involved in the regulation of protein and glycoprotein synthesis in and the release of high molecular weight (mucous) glycoproteins from pig gastric non‐parietal cells and that histamine may be a physiological activator of this system.
Glycoprotein and protein synthesis in and release from pig isolated, enriched gastric mucous cells were measured by the incorporation of N‐acetyl‐[14C]‐d‐glucosamine and [3H]‐l‐leucine, respectively, into cellular and released acid precipitable material.
Histamine and activators of the adenosine 3′:5′‐cyclic monophosphate (cyclic AMP) system maximally stimulated total protein and glycoprotein synthesis in and release from the cells at concentrations of histamine (10 μm), forskolin (10–100 μm), 3‐isobutyl‐1‐methylxanthine (100 μm), and dibutyryl cyclic AMP (1–3 mm), respectively. In the presence of 3‐isobutyl‐1‐methylxanthine (30 μm) histamine stimulation was enhanced.
As shown by gel chromatography, stimulation by histamine (100 μm), forskolin (10 μm), 3‐isobutyl‐1‐methylxanthine (100 μm) and dibutyryl cyclic AMP (1 mm) resulted in a release of high molecular weight (≅2 × 106daltons) glycoproteins from the cells. The histamine H2‐receptor antagonist, ranitidine (100 μm), blocked the effect of histamine.
We conclude that cyclic AMP‐dependent processes are involved in the regulation of protein and glycoprotein synthesis in and the release of high molecular weight (mucous) glycoproteins from pig gastric non‐parietal cells and that histamine may be a physiological activator of this system.
DOI: 10.1111/j.1476-5381.1991.tb12462.x
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