Article date: March 1991
By: Kazuhide Inoue, Ken Nakazawa, Mica Ohara‐Imaizumi, Tomoko Obama, Kannosuke Fujimori, Akira Takanaka, in Volume 102, Issue 3, pages 581-584
Suramin, a putative P2‐antagonist, (10 to 300 μm) inhibited the adenosine 5′‐triphosphate (ATP)‐stimulated secretion of [3H]‐noradrenaline or endogenous dopamine from phaeochromocytoma PC12 cells in a concentration‐dependent manner. Suramin (300 μm) did not affect the dopamine‐secretion stimulated by high K+ or nicotine.
Suramin shifted the concentration‐response curve for ATP to the right. The antagonism was competitive with a pA2 value of 4.52.
ATP also stimulated an increase in intracellular Ca2+ concentration as determined by fura‐2 methods. Suramin antagonized this effect over the same concentration range that antagonized the ATP‐stimulated catecholamine secretion.
These results suggest that suramin can be used as a selective and competitive antagonist of ATP in experiments concerning mechanisms of catecholamine‐secretion.
Suramin, a putative P2‐antagonist, (10 to 300 μm) inhibited the adenosine 5′‐triphosphate (ATP)‐stimulated secretion of [3H]‐noradrenaline or endogenous dopamine from phaeochromocytoma PC12 cells in a concentration‐dependent manner. Suramin (300 μm) did not affect the dopamine‐secretion stimulated by high K+ or nicotine.
Suramin shifted the concentration‐response curve for ATP to the right. The antagonism was competitive with a pA2 value of 4.52.
ATP also stimulated an increase in intracellular Ca2+ concentration as determined by fura‐2 methods. Suramin antagonized this effect over the same concentration range that antagonized the ATP‐stimulated catecholamine secretion.
These results suggest that suramin can be used as a selective and competitive antagonist of ATP in experiments concerning mechanisms of catecholamine‐secretion.
DOI: 10.1111/j.1476-5381.1991.tb12216.x
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