Article date: June 2013
By: Yvonne Mårde Arrhén, Hanna Nylén, Anita Lövgren‐Sandblom, Kajsa P. Kanebratt, Katarina Wide, Ulf Diczfalusy, in Volume 75, Issue 6, pages 1536-1540
Aim
To compare plasma 4β‐hydroxycholesterol : cholesterol with urinary 6β‐hydroxycortisol : cortisol as markers of cytochrome P4503A4 activity before and after treatment with rifampicin for 2 weeks.
Method
6β‐hydroxycortisol and cortisol were determined by liquid chromatography tandem mass spectrometry and 4β‐hydroxycholesterol was determined by gas chromatography–mass spectrometry in three groups of healthy volunteers.
Results
Induction ratios for 6β‐hydroxycortisol : cortisol were 1.8, 3.9 and 4.5 for 20 mg day−1, 100 mg day−1 or 500 mg day−1 of rifampicin, respectively. The corresponding ratios for 4β‐hydroxycholesterol : cholesterol were 1.5, 2.4 and 3.8.
Conclusions
Plasma 4β‐hydroxycholesterol : cholesterol gave similar induction ratios to urinary 6β‐hydroxycortisol : cortisol.
DOI: 10.1111/bcp.12016
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