The genome‐wide expression profile of Curcuma longa‐treated cisplatin‐stimulated HEK293 cells

Article date: October 2010

By: Sung‐Hwa Sohn, Eunjung Ko, Hwan‐Suck Chung, Eun‐Young Lee, Sung‐Hoon Kim, Minkyu Shin, Moochang Hong, Hyunsu Bae, in Volume 70, Issue 4, pages 547-556

WHAT IS ALREADY KNOWN ABOUT THIS SUBJECT

AIM

The rhizome of turmeric, Curcuma longa (CL), is a herbal medicine used in many traditional prescriptions. It has previously been shown that CL treatment showed greater than 47% recovery from cisplatin‐induced cell damage in human kidney HEK 293 cells. This study was conducted to evaluate the recovery mechanisms of CL that occur during cisplatin induced nephrotoxicity by examining the genome wide mRNA expression profiles of HEK 293 ‐cells.

METHOD

Recovery mechanisms of CL that occur during cisplatin‐induced nephrotoxicity were determined by microarray, real‐time PCR, immunofluorescent confocal microscopy and Western blot analysis.

RESULTS

The results of microarray analysis and real‐time PCR revealed that NFκB pathway‐related genes and apoptosis‐related genes were down‐regulated in CL‐treated HEK 293 cells. In addition, immunofluorescent confocal microscopy and Western blot analysis revealed that NFκB p65 nuclear translocation was inhibited in CL‐treated HEK 293 cells. Therefore, the mechanism responsible for the effects of CL on HEK 293 cells is closely associated with regulation of the NFκB pathway.

CONCLUSION

CL possesses novel therapeutic agents that can be used for the prevention or treatment of cisplatin‐induced renal disorders.

DOI: 10.1111/j.1365-2125.2010.03724.x

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