In vitro and in vivo evaluation of the inhibition potential of risperidone toward clozapine biotransformation

Article date: October 2009

By: Manoranjenni Chetty, Fabrizio D'Esposito, Wei V. Zhang, John Glen, Glenys Dore, Zvijezdana Stankovic, Robert J. Edwards, Iqbal Ramzan, Michael Murray, in Volume 68, Issue 4, pages 574-579

WHAT IS ALREADY KNOWN ABOUT THIS SUBJECT

AIMS

To study the impact of risperidone (RISP) on clozapine (CLZ) biotransformation in vitro in microsomal fractions containing varying expression of CYP oxidases and in vivo in patients.

METHODS

Human liver microsomes (n= 11) were assessed for expression of CYPs 1A2, 2D6 and 3A4, because these enzymes mediate RISP and CLZ oxidation. Inhibition of CLZ oxidation by RISP was assessed. Plasma CLZ elimination was estimated in patients with schizophrenia who received either CLZ alone or the CLZ–RISP combination (n= 10 per group).

RESULTS

(i) The CYP3A4 and CYP1A2 inhibitors ketoconazole and fluvoxamine inhibited CLZ oxidation to varying extents in individual microsomal fractions. (ii) RISP did not inhibit CLZ oxidation, regardless of variations in CYP expression. (iii) RISP co‐administration did not impair CLZ clearance.

CONCLUSIONS

No evidence was found for CYP‐mediated inhibitory or pharmacokinetic interactions between RISP and CLZ. Occasional literature reports of such interactions may involve other pathways that participate in CLZ disposition.

DOI: 10.1111/j.1365-2125.2009.03476.x

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