Article date: December 1963
By: R. LAVERTY, I. A. MICHAELSON, D. F. SHARMAN, V. P. WHITTAKER in Volume 21, Issue 3, pages 482-490
The distribution of dopamine (3,4‐dihydroxyphènylethylamine) between the subcellular fractions from homogenates of dog caudate nucleus was compared with the distributions of acetylcholine, 5‐hydroxytryptamine and lactate dehydrogenase activity. The distributions of noradrenaline and 5‐hydroxytryptamine between the subcellular fractions from homogenates of dog hypothalamus were also determined. Most of the dopamine, in contrast to acetylcholine, occurred in the soluble supernatant fraction; the remainder was associated with the fractions rich in pinched‐off nerve ending particles, but localization in any one fraction was not as sharp as that of acetylcholine. Evidence is presented that suggests that the dopamine occurs in a free or easily released form throughout cell cytoplasm.
DOI: 10.1111/j.1476-5381.1963.tb02016.x
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